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2× HotStart Taq PCR MasterMix is a 2× concentrated, optimized mixture composed of HotStart Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The advantages of 2×HotStart PCR MasterMix include high convenience, sensitivity, specificity and stability. It minimizes man-made errors during PCR operating process. HotStart inhibitor blocks the substrate binding site of HotStart Taq DNA polymerases in a temperature-dependent manner. Inactive polymerase-inhibitor complexes are formed at temperatures < 40°C, where the affinity of HotStart inhibitor for HotStart Taq DNA polymerase is higher than the binding affinity of the template DNA. Between 40°C and 55°C the HotStart inhibitor competes with the template DNA for binding to the Taq DNA polymerase, thereby shifting the binding equilibrium towards complex formation with only target-specific primed template DNA. This minimizes the non-specific amplification in PCR and ensures high sensitivity and specificity.